Acta Orthopaedica et Traumatologica Turcica
Research Articles

Investigation into the effect of epidermal growth factor and hyaluronic acid on fracture healing in a rat femoral fracture model

1.

Department Orthopaedics and Traumatology, Çerkezköy State Hospital, Tekirdağ, Turkey

2.

Department Orthopaedics and Traumatology, Hacettepe University, Ankara, Turkey

3.

IRCCS Istituto Ortopedico Galeazzi, Milan, Italy

4.

Department Thoracic Surgery, Medical Sciences University, Gülhane Training and Research Hospital, Ankara, Turkey

5.

Department of Medicine, Surgery and Dentistry, University of Salerno, Via S. Allende, Baronissi, Italy

6.

Department Orthopaedics and Traumatology, Medical Park Hospital, Antalya, Turkey

AOTT 2023; 57: 229-236
DOI: 10.5152/j.aott.2023.22163
Read: 677 Downloads: 264 Published: 25 October 2023

Objective: This study aimed to investigate the role of an exogenous Epidermal Growth Factor and a hyaluronic acid-based scaffold on fracture healing in a rat femoral fracture model

Methods: Forty-eight male Wistar-Albino rats, each weighing a mean 392 grams (range= 350-450 grams) and aged 8.2 months (6-9 months), were used for this experimental study. All surgical procedures were performed on the left femur by a single surgeon. An open femoral fracture was created in all rats. The animals were randomly divided into one of the four groups: Control (12), EGF (12), HA (12) and Combined (12). In the 4th and sixth weeks, samples were processed and analyzed using biomechanical and histological methods.

Results: Fracture healing was significantly improved in the Combined group compared to the control one, EGF and HA groups in all parameters at both experimental time points. At the fourth and sixth weeks after surgery, fracture healing in the EGF and HA groups was significantly increased at histological evaluation compared to controls. In addition, compared with EGF, HA and Control groups, a significant difference in callus tissue was detected in the Combined group at 4 and 6-week time points in biomechanical features.

Conclusion: This study has shown that combining local EGF and HA scaffold accelerates bone healing and strengthens the bony callus histologically and biomechanically. Using EGF-HA combined scaffolds may represent a possible future strategy in trauma surgery.

Level of Evidence: N/A.

Cite this article as: Kuzuca BC, Doral MN, Mangiavini L, Sapmaz E, Maffulli N, Koken M. Investigation into the effect of epidermal growth factor and hyaluronic acid on fracture healing in a rat femoral fracture model. Acta Orthop Traumatol Turc., 2023;57(5):229-236.

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ISSN 1017-995X EISSN 2589-1294