Effect of plateletrich plasma for treatment of Achilles tendons in freemoving rats after surgical incision and treatment
Purpose: The purpose of this study is to investigate, both histopathologically and biomechanically, the effect of platelet rich plasma (PRP) on surgically repaired Achilles tendon rupture recovery in rats
Materials and Methods: The study included 17-month-old 25 male rats of Sprague Dawley type, with a weight average of 500-550 gr. 5 rats were used as donors while 20 rats were separated as a PRP group (n:10) and Control group (n:10). The Achilles tendons of the rats were cut transversely and PRP was administered to the PRP group, while the control group received serum physiologically to create the same surgical effect. In in previous studies, it was shown that this serum does not have any effect on tendon recovery. 15 and 30 days after the treatment, the rats were sacrificed and their Achilles tendons were extracted and examined histopathologically and biomechanically.
Results: Based on the obtained findings, it was observed that the histopathologic Movin and Bonar scores of PRP group in 15th and 30th day were significantly lower than control group (p=0.019, p=0.017) while no significant difference was found in maximum force (Fmax) values in biomechanical measures in 15th and 30th day.
Conclusions: It is thought that PRP use in Achilles tendon ruptures positively affects histopathological recovery in early period but that it does not reflect the same effect biomechanically. We believe that PRP use for qualified tendon recovery is beneficial histopathologically.
Keywords: Platelet rich plasma, Achilles tendon injuries, Rat, Histopathology, Biomechanics