The effect of doxorubicin on skeletal muscle: an experimental study

Emre Cullu, Ilhan Ozkan, Nil Cuhaci, Bulent Alparslan, Emel Dikicioglu, S. Oner Savk,

Abstract

Objectives: The spasticity-solving effect of botulinum toxin is utilized in the conservative treatment of involuntary and uncontrolled muscle diseases. However, this effect is about of three-month duration, with reasonably high cost. Doxorubicin is a cheap antineoplastic agent reported to cause muscle necrosis. This experimental study sought to investigate the effect of doxorubicin on the skeletal muscle.\nMethods: Twelve male Wistar rats were assigned to doxorubicin injection of 0.02 mg/ 0.5 ml (n=6) and 0.05 mg/ 0.5 ml (n=6) into the left quadriceps muscles through a skin incision of one centimeter under general anesthesia. The right quadriceps muscles of the same rats were assigned as controls and 0.5 ml 0.9% NaCl solution was injected. All the rats were sacrificed under high dose anesthesia seven days later and changes in the muscle tissue were evaluated under light microscope. \nResults: Histopathologically, doxorubicin injection of 0.02 mg resulted in a mean of 3% muscle necrosis and slight inflammation, whereas with 0.05 mg doxorubicin, muscle necrosis was 11% and moderate to severe inflammation was noted. Histopatological findings were normal in control muscles.\nConclusion: With its chemomyectomy effect, doxorubicin may open new horizons in the treatment of spasticity in orthopaedics, without the need for surgical intervention.

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